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HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Express C18

HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 µm particles (53814-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile; (85:15, A:B)
flow rate 1.2 ml/min
sample 200 ng/mL in 25:75, water: acetonitrile
injection 4 µl
detector UV, 254 nm

Description

Analysis Note This application demonstrates the suitability of the Ascentis Express C18 for the analysis of fluoroquinolones.
Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Food and Beverages
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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