HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column

HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column

Conditions

column BIOshell A160 Pejptide C18, 15 cm x 4.6 mm, 5 µm particles (67015-U)
column temp. 60 °C
mobile phase [A] Water with 0.1% TFA, [B] Acetonitrile with 0.1% TFA
gradient 28-55% B in 5 min
flow rate 1.5 mL/min
pressure 2465 psiTotally Porous (170 bar)
pressure 1378 psiBIOshell (95 bar)
sample Water with 0.1% TFA
injection 15 µL
detector UV, 280 nm

Description

Analysis Note The 5μm BIOshell 160Ã… Peptide C18 column offers increased resolution, faster separations, and lower back pressure when compared to a totally porous 3μm C18 column. In addition, the BIOshell column offers a level of ruggedness that is not often associated with columns exhibiting such a high level of performance.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links