HPLC Analysis of Furosemide (Lasix) in Horse Serum on Discovery® C18 after SPE using Discovery® DSC-18

HPLC Analysis of Furosemide (Lasix) in Horse Serum on Discovery® C18 after SPE using Discovery® DSC-18

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix acidified horse serum spiked with indapamide at 10 mg/mL
SPE tube/cartridge Discovery DSC-18, 50 mg/1 mL (52601-U)
condition 1 mL methanol; 1 mL 10 mM potassium phosphate, pH 3
sample addition 1 mL
washing 10 mM potassium phosphate, pH 3
elution 1 mL 60% methanol in DI water.
column Discovery C18, 15 cm x 4.6 mm I.D., 5 µm particles (504955)
column temp. 35 °C
mobile phase [A] 10 mM KH2PO4, pH 3 (adjusted with H3PO4): [B] acetonitrile; (60:40, A:B)
flow rate 1 mL/min
injection 10 µL
detector UV, 234 nm

Description

Application Sample Pre-treatment:
Neat horse serum was acidified with 10 mL 6 M HCl/mL serum. Internal standard (I.S.) blank was prepared by spiking acidified horse serum with indapamide at 10 mg/mL. Sample A = 10 mg/mL furosemide in I.S. blank. Sample B = 5 mg/mL furosemide in I.S. blank. Sample C = 0.5 mg/mL furosemide in I.S. blank. Sample D = 0.1 mg/mL furosemide in I.S. blank. Sample E = 0.05 mg/mL furosemide in I.S. blank.
Categories Analytical Chromatography, SPE Applications, Forensics and Toxicology, Pharmaceutical & Clinical applications, diuretics, Diuretics
Featured Industry Forensics and Toxicology
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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