HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl

HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl

Conditions

column Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 µm particles (53336-U)
column temp. 30 °C
mobile phase [A] water, acetonitrile, 90:10; [B] acetonitrile
gradient 0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min
flow rate 0.3 mL/min
pressure 192 bar (2780 psi)
sample 5 mg/L ea. in 95:5, water:acetonitrile
injection 10 µL
detector UV, 218 nm

Description

Analysis Note The trichothecene vomitoxins of Fusarium are significant plant pathogens of grain and forage crops that can lead to anorexic effects with livestock. This application demonstrates resolution of deoxynivalenol and three of its analogs.
Categories Analytical Chromatography, Mycotoxins
Featured Industry Agriculture
Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links