HPLC Analysis of Fusion Protein on Zenix®-C SEC-300 vs. Zenix® SEC-300

HPLC Analysis of Fusion Protein on Zenix®-C SEC-300 vs. Zenix® SEC-300

Conditions

column Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 µm particles (Z777092)
column Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µm particles (Z777033)
column temp. 25 °C
mobile phase 150 mM sodium phosphate buffer, pH 7.0 + 200 mM NaCl
flow rate 0.5 mL/min
sample 1 mg/mL fusion protein, MW 170 kD, pl 6.8-7.0
injection 10 µL
detector UV, 214 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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