HPLC Analysis of Fusion Protein on Zenix®-C SEC-300, Effect of Mobile Phase Composition

HPLC Analysis of Fusion Protein on Zenix®-C SEC-300, Effect of Mobile Phase Composition

Conditions

column Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 µm particles (Z777092)
column temp. 25 °C
gradient (A) lower, 150 mM sodium phosphate buffer, pH 7.0 (B) middle, 150 mM sodium phosphate buffer, pH 7.0 + 10% acetonitrile (C) top, 150 mM sodium phosphate buffer, pH 7.0 + 20 mM NaCl
flow rate 0.5 mL/min
sample 1 mg/mL fusion protein, MW 170 kD, pl 6.8-7.0
injection 10 µL
detector UV, 214 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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