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HPLC Analysis of Ginsenosides on Ascentis® Express C18

HPLC Analysis of Ginsenosides on Ascentis® Express C18

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 5 µm particles (50537-U)
column temp. 60 C
mobile phase [A] water, [B] acetonitrile
gradient 25% B held for 3 min; to 85% B in 12 min;85% B held for 1 min
flow rate 1 ml/min
pressure 1470 psi (101 bar)
sample 50 µg/mL in 82:18, water:acetonitrile
injection 5 µl
detector UV, 205 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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