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HPLC Analysis of Glucocorticoids on Ascentis® C8

HPLC Analysis of Glucocorticoids on Ascentis® C8


column Ascentis C8, 15 cm x 4.6 mm I.D., 5 µm particles (581424-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (68:32, A:B)
flow rate 1.0 ml/min
sample as indicated in mobile phase
injection 10 µl
detector UV, 254 nm


Categories Analytical Chromatography, Clinical, Steroids, hormones, steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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