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HPLC Analysis of Grapefruit Juice on Ascentis® Express C18 Column

HPLC Analysis of Grapefruit Juice on Ascentis® Express C18 Column

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix grapefruit juice hand-squeezed in laboratory, centrifuged and filtered through a 0.45 µm nylon filter disc (Acrodisc)
column Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 µm particles (53826-U)
column temp. ambient
mobile phase [A] water/formic acid (99.9:0.1); [B] water/acetonitrile/isopropanol/formic acid (39.9:20:40:0.1)
gradient 10% B for 3 min; 10 to 34% B in 30 min; 34 to 100% B in 1 min; held at 100% B for 6 min; 100 to10% B in 1 min
flow rate 0.8 ml/min
injection 2 µl
detector ESI-MS: mass spectral range, 250-700 m/z; interval, 0.5 s; scan speed, 938 amu/s; nebulizing gas (N2) flow, 1.5 L/min; interface temperature, 350 C; (heat block, 300 C; desolvation line (DL) temperature, 300 C; DL voltage, 34 V; probe voltage, +4.5 kV; Qarray voltage, 1.0 V; RF voltage, 90 V; detection gain, 1.05 kV.)
detector PDA: 190-370 nm; chromatogram extracted at 283 nm

Description

Categories Analytical Chromatography, SPE Applications, Food & Beverage Analysis, Flavors/fragrances, Flavors, Fragrances, Beverage Testing, Fruit Juice
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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