HPLC Analysis of Hae III Digest of pBR22 DNA on TSKgel® NPR, High Resolution, Fast Analysis

HPLC Analysis of Hae III Digest of pBR22 DNA on TSKgel® NPR, High Resolution, Fast Analysis

Conditions

column TSKgel DEAE-NPR gaurd column, 0.5 cm x 4.6 mm I.D., 5 µm particles (817088)
column TSKgel DEAE-NPR, 3.5 cm x 4.6 mm I.D., 2.5 µm particles (813075)
column temp. 40 °C
mobile phase [A] 0.02 M Tris-HCl, pH 9.0; [B] 0.02 M Tris-HCl, 1.0 M NaCl, pH 9.0
gradient 48 to 65% B in 15 min
flow rate 1.5 mL/min
pressure 2000 psi138 bar
sample Hae III Digest of pBR22 DNA, base pair number for each peak is indicated on the figure
detector UV, 260 nm

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
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