HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX

HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX

Conditions

column Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 µm particles (59602-U)
column temp. 35 °C
mobile phase [A] 10 mM Tris, pH 8.0 with acetic acid; [B] 10 mM Tris, 0.25 M KCl, pH 8.0 with acetic acid
gradient 0 to 32% B in 20 min. (linear)
flow rate 0.5 mL/min
injection 50 mug each Hb variants (A2, S and A0)
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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