HPLC Analysis of Hippuric and Methylhippuric Acids in Urine on SUPELCOSIL™ LC-18

HPLC Analysis of Hippuric and Methylhippuric Acids in Urine on SUPELCOSIL™ LC-18

Conditions

column SUPELCOSIL LC-18, 7.5 cm x 4.6 mm I.D., 3 µm particles (58984)
mobile phase [A] tetrahydrofuran:10 mM potassium phosphate, pH 3.0 [B] with phosphoric acid (3:97, A:B)
flow rate 1.5 ml/min
injection 10 µl spiked urine (300 µg/mL HA, o-MHA; 150 µg/mL m-MHA, p-MHA)
detector UV, 260 nm

Description

Analysis Note Urinary excretion of hippuric acid and m-or p-methylhippuric acid in the urine is a test of exposure to toluene and m-or p-xylene vapors.
Categories Analytical Chromatography, Clinical, Forensics and Toxicology, organic acids, Organic Acids
Featured Industry Clinical
Forensics and Toxicology
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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