HPLC Analysis of Horny Goat Weed Extract on Ascentis® Express C18

HPLC Analysis of Horny Goat Weed Extract on Ascentis® Express C18

Conditions

sample preparation to 100 mg of Horny Goat Weed herbal supplement add 10 mL ethanol, sonicate for 30 min, centrifuge 10,000 rpm for 1 min, filter supernatant using 0.45 µm filter, dilute to 30:70, water:ethanol
column Ascentis Express C18, 15 cm x 4.6 mm I.D., 5 µm particles (50537-U)
column temp. 35 C
mobile phase (A) 0.1% acetic acid in water; (B) 0.1% acetic acid in acetonitrile;
gradient 5% B for 0.75 min; to 40% B in 3.75 min; to 100% B in 3.5 min; held at 100% B for 2 min
flow rate 1.5 ml/min
pressure 2120 psi (146 bar)
sample each compound, 50 µg/mL in water:methanol (70:30)
injection 5 µl
detector UV, 250 nm

Description

Categories Analytical Chromatography, ED Drugs
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links