HPLC Analysis of Horse Serum Proteins in Acetonitrile as a Function of Ammonium Formate Concentration

HPLC Analysis of Horse Serum Proteins in Acetonitrile as a Function of Ammonium Formate Concentration

Conditions

column SRT SEC-150, 30 cm x 4.6 mm I.D., 5 µm particles, 150 Ã… (Z777043)
mobile phase 100-200 mM CH3COONH4/CH3CN, pH 6.3
flow rate 0.25 mL/min
sample Molecular weight marker proteins, horse serum
detector SofTA ELSD

Description

Analysis Note Although horse serum proteins can be analyzed under standard SEC conditions, the resolution is poor. However, very high efficiency separations can be achieved when using pure acetonitrile to which ammonium formate has been added. About 200 mM ammonium formate is required for all proteins to elute within the pore volume of SRT SEC-150 column. The position of the components can be modulated by decreasing the ammonium formate concentration.
Ref.: V. McKay; X. Huang, LC-GC North America;Sep2006 Supplement, Vol. 24, p25, 2D Chromatography of Proteins: Horse Serum Analysis
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Other Notes Courtesy of Miyako Kawakatsu, M&S Instruments Inc.
suitability application for HPLC

Materials

     
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