HPLC Analysis of Insulin (Species Variants) on BIOshell™ A160 Peptide C18

HPLC Analysis of Insulin (Species Variants) on BIOshell™ A160 Peptide C18

Conditions

column BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 µm particles (66905-U)
column temp. 30 C
mobile phase [A] 70:30, water with 0.1% TFA:acetonitrile with 0.09% TFA; [B] 55:45, water with 0.1% TFA:acetonitrile with 0.09% TFA
gradient 0 to 100% B in 7.5 min
flow rate 0.3 ml/min
pressure 4040 psi (278 bar)
sample 200 µg/mL insulin (bovine, human, porcine) in 0.1% formic acid
injection 0.5 µl
detector UV, 215 nm

Description

Analysis Note These three insulins have more than 76% identical positions in a primary structure alignment. The human and porcine proteins are most similar with 85% identity. Baseline resolution of these insulins in achieved on BIOshell A160 Peptide C18, with relative band spacing of these three peaks approximating their relative sequence similarities. BIOshell A160 Peptide C18 is ideally suited for RP chromatography of small proteins or large peptides.
Categories Analytical Chromatography, Proteins, Peptides, Hormones
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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