Certain features of will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Isocyanate Derivatives on Ascentis® ES-Cyano

HPLC Analysis of Isocyanate Derivatives on Ascentis® ES-Cyano


column Ascentis Cyano, 15 cm x 4.6 mm I.D., 5 µm particles (577306-U)
column temp. 35 C
mobile phase [A] 0.1% ammonium acetate in water; [B] 0.1% ammonium acetate in acetonitrile; (70:30, A:B)
flow rate 1.0 ml/min
sample 20 µg/mL in 80:12:8, water: acetonitrile: dimethylsulfoxide
injection 5 µl
detector UV, 254 nm


Analysis Note This application demonstrates the suitability of Ascentis Cyano for the efficient separation of isocyanate derivatives.
Categories Analytical Chromatography, Isocyanates
Featured Industry Industrial Hygiene
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


Related Links