Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Levothyroxine and Liothyronine on Ascentis® Express ES-Cyano

HPLC Analysis of Levothyroxine and Liothyronine on Ascentis® Express ES-Cyano

Conditions

column Ascentis Express ES-Cyano, 10 cm x 2.1 mm I.D., 2.7 µm particles (53491-U)
column temp. 30 C
mobile phase [A] water with 0.05% phosphoric acid; [B] acetonitrile with 0.05% phophoric acid; (60:40, A:B)
flow rate 1.5 ml/min
pressure 3270 psi (225 bar)
sample 20 µg/mL in mobile phase
injection 5 µl
detector UV, 225 nm

Description

Analysis Note This application demonstrates the suitabilty of Ascentis Express ES-Cyano for the analysis of Levothyroxine according to USP Monograph 3301 with Liothyronine as a reference standard.
Categories Analytical Chromatography, Clinical, Hormones, hormones
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links