HPLC Analysis of mAb 321 on Proteomix® SCX NP5 versus Competitive SCX using pH and Salt Gradients

HPLC Analysis of mAb 321 on Proteomix® SCX NP5 versus Competitive SCX using pH and Salt Gradients

Conditions

column Proteomix SCX-NP5, 25 cm x 4.6 mm I.D. (PEEK hardware), 5 µm particles (Z777164)
mobile phase (A) 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; (B) A + 0.5 M NaCl, pH 10.5
flow rate 0.8 ml/min
sample MAb 321 (5 mg/mL)
injection 20 µl
detector UV, 280 nm

Description

Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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