HPLC Analysis of MAb on Proteomix® SCX-NP5 Comparing Different Buffer Systems and pH Gradients

HPLC Analysis of MAb on Proteomix® SCX-NP5 Comparing Different Buffer Systems and pH Gradients

Conditions

column Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 µm particles (Z777161)
mobile phase Mobile phase A: (A) 20 mM MES, pH 6.0; (B) A + 1 M NaCl, pH 6.0Mobile phase B: (A) 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; (B) A, pH 10.5
gradient Gradient A: 7% B held for 2 min; to 14% B in 20 min, Gradient B: 0 to 30% B in 2 min; to 55% B in 30 min
flow rate 0.8 ml/min
sample 10 mg/mL
injection 5 µl
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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