HPLC Analysis of mAb-X22 on Antibodix® WCX-NP10, Effect of Gradient Profile

HPLC Analysis of mAb-X22 on Antibodix® WCX-NP10, Effect of Gradient Profile

Conditions

column Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 µm particles (Z777272)
mobile phase (A) 10 mM sodium phosphate buffer, pH 7.5; (B), A + 100 mM NaCl
gradient (A) 15-75% B in 30 min(B) 15-65% B in 30 min(C) 15-55% B in 30 min(D) 15-47.5% B in 30 min(E) 15-40% B in 30 min and 40% B after 30 min
flow rate 0.8 ml/min
temp. 25 C
sample MAb-X22, 1.5 mg/mL
injection 10 µl
detector UV, 214 nm

Description

Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Life Science and Biopharma
Legal Information Antibodix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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