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HPLC Analysis of Medium MW Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size

HPLC Analysis of Medium MW Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size

Conditions

column Zenix SEC-150, 30 cm x 4.6 mm I.D., 3 µm particles (Z777016)
column Zenix SEC-100, 30 cm x 4.6 mm I.D., 3 µm particles (Z777006)
column Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm particles (Z777028)
column temp. 25 C
mobile phase 0.2 M sodium phosphate dibasic, pH 7.0 with phosphoric acid
flow rate 0.25 ml/min
pressure 1000 psi (69 bar)
sample Waters BEH200 protein standard
injection 0.5 µl
detector UV, 215 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
General description Pore size is a kay parameter of resolution size exclusion chromatography. Matching the pore size to resolution of the sample components is critical in selecting a column. This application shows the impact of pore size on resolution of sample components as a function of molecular size
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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