HPLC analysis of Methyl Paraben and Organic Acids on Ascentis® Express RP-Amide

HPLC analysis of Methyl Paraben and Organic Acids on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (50774-U)
column temp. 25 °C
mobile phase [A] 0.1% formic acid; [B] acetonitrile; (70:30, A:B)
flow rate 1.3 mL/min
pressure 1870 psi (129 bar)
sample [A] water; [B] acetonitrile; (50:50, A:B)
injection 5 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Antifungals
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Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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