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HPLC Analysis of Norphenylephrine Enantiomers on Astec® CYCLOBOND® I 2000 AC (pH 5.0)

HPLC Analysis of Norphenylephrine Enantiomers on Astec® CYCLOBOND® I 2000 AC (pH 5.0)

Conditions

column CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 µm particles (20124AST)
column temp. 25 C
mobile phase [A] methanol; [B] 20 mM ammonium acetate, pH 5.0; (5:95, A:B)
flow rate 0.5 ml/min
sample norphenylephrine, 1 mg/mL in acetonitrile:water (50:50)
injection 5 µl
detector UV, 230 nm

Description

Categories Analytical Chromatography, Amines
Featured Industry Pharmaceutical (small molecule)
Legal Information Astec is a registered trademark of Sigma-Aldrich Co. LLC, CYCLOBOND is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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