HPLC Analysis of Nucleotides on SUPELCOSIL™ LC-18-T

HPLC Analysis of Nucleotides on SUPELCOSIL™ LC-18-T

Conditions

column SUPELCOSIL LC-18-T, 15 cm x 4.6 mm I.D., 3 µm particles (58970-U)
mobile phase [A] 0.1M potassium phosphate buffer/4mM tertrabutylammonium hydrogen sulfate, pH6.0; [B] A:methanol (70:30), pH 7.2
flow rate 1.5 mL/min
injection 20 µL deionized water (25-530 µm each analyte)
detector UV, 254 nm

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Life Science and Biopharma
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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