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HPLC Analysis of Organic Acids on Ascentis® C8

HPLC Analysis of Organic Acids on Ascentis® C8


column Ascentis C8, 15 cm x 4.6 mm I.D., 5 µm particles (581424-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium phosphate monobasic (pH 2.50 with phosphoric acid): [B] acetonitrile; (80:20, A:B)
flow rate 1.0 ml/min
sample as indicated in mobile phase
injection 10 µl
detector UV, 220 nm


Categories Analytical Chromatography, Organic Acids
Featured Industry Chemicals and Industrial Polymers
Food and Beverages
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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