HPLC Analysis of Organic Acids on Ascentis® RP-Amide

HPLC Analysis of Organic Acids on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 10 cm x 2.1 mm I.D., 3 µm particles (565301-U)
column temp. 35 °C
mobile phase [A] 0.1% (v/v) TFA in [B] water
flow rate 0.3 mL/min
sample 0.2 - 4 g/L in mobile phase
injection 1 µL
detector UV, 210 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis RP-Amide for the analysis of organic acids under 100% aqueous conditions.
Categories Analytical Chromatography, Organic Acids
Featured Industry Chemicals and Industrial Polymers
Food and Beverages
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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