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HPLC Analysis of Paclitaxel and Related Compounds on Ascentis® Express F5

HPLC Analysis of Paclitaxel and Related Compounds on Ascentis® Express F5


column Ascentis Express F5, 15 cm x 4.6 mm I.D., 5 µm particles (50631-U)
column temp. 30 C
mobile phase [A] water; [B] acetonitrile; (60:40, A:B)
flow rate 1.5 ml/min
pressure 2727 psi (188 bar)
sample taxols 25 μg/mL in 70:15:15, water:methanol:acetonitrile
injection 5 µl
detector UV, 227 nm


Analysis Note An efficient reverse phase HPLC method for the HPLC analysis of of Paclitaxel (Taxol) and related compounds is developed using the Ascentis® Express F5 5μm Fused Core HPLC column. Paclitaxel is a natural product with antitumor activity. TAXOL (paclitaxel) is obtained via a semi-synthetic process from Taxus baccata.
The developed method can be easily used for the routine quality control of bulk and Parenterals dosage form of Paclitaxel within a short analysis time. This method offers the advantages of higher efficies, reduced backpressure and shorter run times over a traditionally run methods over 5 μm totally porous particle columns.
Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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