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HPLC Analysis of PAHs on SUPELCOSIL™ LC-PAH

HPLC Analysis of PAHs on SUPELCOSIL™ LC-PAH

Conditions

column SUPELCOSIL LC-PAH, 15 cm x 4.6 mm I.D., 5 µm particles (58318)
column temp. 35 C
mobile phase [A] water, [B] methanol
gradient 55 to 100% B in 5 min; held at 100%B for 10 min
flow rate 1.5 ml/min
sample EPA 610 mix
injection 5 µl
detector UV, 254 nm

Description

Analysis Note PAHs are traditionally evaluated using an acetonitrile/water gradient. Recently customers are looking for alternatives to this method using a methanol/water gradient due to the risk of an another acetonitrile shortage, solvent costs, and toxicity to the environment. This analysis demostrates the separation using a methanol gradient.
Categories Analytical Chromatography, Environmental Analysis, Hydrocarbons, Organics, Chromatograms, HPLC Chromatograms
Featured Industry Environmental
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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