HPLC Analysis of PAHs in Canola Oil on SUPELCOSIL™ LC-PAH after SPE using Supelclean™ EZ-POP NP

HPLC Analysis of PAHs in Canola Oil on SUPELCOSIL™ LC-PAH after SPE using Supelclean™ EZ-POP NP

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix Canola oil spiked with PAHs at 10 ng/g
SPE tube/cartridge Supelclean(TM) EZ-POP NP, 12 mL, pk of 20 (54341-U)
condition 10 mL acetone, dry under 10-15" mercury for 10 minutes
sample addition 0.5 mLweighed accurately onto cartridge
elution 15 mLacetonitrile
eluate post-treatment concentrate to final volume of 0.5mL at 40 C under nitrogen
column SUPELCOSIL(TM) LC-PAH 25 cm x 4.6 mm I.D., 5 µm particles (58229)
column temp. 25 C
mobile phase [A] water [B] acetonitrile
gradient 40% B held for 5 min; to 100% B in 15 min; held at 100% B for 12 min
flow rate 2.4 ml/min
pressure 2790 psiat start (192 bar)
detector Fluorescence, programmed.

Description

Analysis Note PAHs are ubiquitous in the environment, resulting from both natural and manmade sources of combustion. They can contaminate food crops through environmental exposure to particulates, automobile exhaust, etc. In the case of edible oils, additional PAHs can be introduced during actual production processes such as seed drying [1]. In this application, EZ-POP NP SPE is used to extract PAHs from soybean oil. The 16 PAHs designated in many US EPA methods (less acenaphthylene) were analyzed by HPLC-FLD after extraction from a spiked sample of oil.
Categories Analytical Chromatography, SPE Applications, PAHs
Featured Industry Food and Beverages
Legal Information Supelclean is a trademark of Sigma-Aldrich Co. LLC, SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Other Notes Detector: Naphthalene, Acenaphthene, Fluorene - 225nm/320nm; Phenanthrene, Anthracene - 250nm/368nm; Fluoranthene, Pyrene - 237nm/440nm; Benzo[a]anthracene, Chrysene - 265nm/380nm; Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[a]pyrene, Dibenzo[a,h]anthracene - 280nm/420nm; Benzo[g,h,i]perylene, Indeno[1,2,3-cd]pyrene, Benzo[b]chrysene - 300nm/466nm
suitability application for SPE, application for HPLC

Materials

     
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