HPLC Analysis of PAHs in Water on SUPELCOSIL™ LC-PAH after SPME using 100 μm PDMS Fiber

HPLC Analysis of PAHs in Water on SUPELCOSIL™ LC-PAH after SPME using 100 μm PDMS Fiber

Conditions

sample/matrix 5 µL PAH mix (Cat. No. 4-8743) in 5 mL water
desorption process static, 200 µL acetonitrile:water, 40:60, 2 min
extraction immersion, 30 min (rapid stirring)
SPME fiber polydimethylsiloxane, 100 µm (57301)
column SUPELCOSIL LC-PAH, 15 cm x 4.6 mm I.D., 5 µm particles (58318)
mobile phase [A] acetonitrile:[B] water
gradient 0 min - 50% A; 5 min - 50% A; 30 min - 100% A; 45 min - 100% A
flow rate 0-2 min: 0.2 mL/min 2-45 min: 1.0 mL/min
detector UV, 254 nm

Description

Categories Analytical Chromatography, Food & Beverage Analysis, Environmental Analysis, SPME Applications, Environmental, Processing/Packaging Contaminants, Organics, Semivolatiles, PAHs, Chromatograms, Applications (Chromatograms), HPLC Chromatograms
Featured Industry Environmental
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for SPME, application for HPLC

Materials

     
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