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HPLC Analysis of Pain Relievers on Ascentis® Express C18

HPLC Analysis of Pain Relievers on Ascentis® Express C18


column Ascentis Express C18, 10 cm x 4.6 mm I.D., 5 µm particles (50536-U)
column temp. 45 C
mobile phase [A] water with 3% acetic acid; [B] methanol with 3% acetic acid; (79:21, A:B)
flow rate 2.0 ml/min
pressure 3290 psi (227 bar)
sample 100 - 360 µg/mLin mobile phase
injection 10 µl
detector UV,275 nm


Analysis Note This application shows the improvements in efficiency that may be obtained for USP methods using modern Fused-Core technology.
Categories Analytical Chromatography, Clinical, analgesics, Analgesics
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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