HPLC Analysis of Paraquat and Diquat on SUPELCOSIL™ LC-18 after SPE using ENVI™-8 DSK

HPLC Analysis of Paraquat and Diquat on SUPELCOSIL™ LC-18 after SPE using ENVI™-8 DSK

Conditions

sample preparation ENVI-8 DSK, 47 mm (57172)
sample preparation SPE (Solid Phase Extraction)
sample/matrix drinking water adjusted to pH to 10.
SPE tube/cartridge ENVI-8 DSK SPE Disk, 47 mm (57172)
condition 10 mL acetonitrile; 2 x 10 mL reagent water; 10 mL conditioning solvent A (5 g cetyl trimethyl ammonium bromide and 5 mL conc. ammonium hydroxide in 500 mL DI water, dilute to 1 L); (2 x 10 mL reagent water; 10 mL conditioning solvent B (10 g hexanesulfonic acid, sodium salt and 10 mL conc. ammonium hydroxide in 250 mL DI water, dilute to 500 mL))
sample addition 250 mL water 100 ml/min
elution 0.5-1.0 mL acetonitrile (to cover/solvate disk); 2 x 4 mL eluting solution (13.5 mL orthophosphoric acid and 10.3 mL diethylamine in 500 mL DI water, dilute to 1 L)
column SUPELCOSIL LC-18, 15 cm x 4.6 mm I.D., 5 µm particles (58230-U)
column temp. 35 C
mobile phase 3.5 mL triethylamine and 1.0 g 1-hexane-sulfonic acid, sodium salt to 800 mL DI H2O add orthophosphoric acid to pH 2.5, dilute to 1 L
flow rate 1.0 ml/min
injection 100 µl
detector photodiode array, diquat - 308 nm, paraquat - 257 nm

Description

Application Refer to US EPA Method 549.1 for full details

Sample Pre-treatment:
250 mL drinking water. Adjust water sample to pH to 10.5 ± 0.2 with 10% sodium hydroxide or 10% hydrochloric acid
Categories Analytical Chromatography, Environmental Analysis, SPE Applications, Food & Beverage Analysis, Pesticides, Beverage Testing, Herbicides, Organics, Drinking Water and Bottled Water, Chromatograms, HPLC Chromatograms
Featured Industry Environmental
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC, ENVI is a trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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