HPLC Analysis of Penicillin G and Phenoxymethylpenicillinic Acid on Ascentis® C18

HPLC Analysis of Penicillin G and Phenoxymethylpenicillinic Acid on Ascentis® C18

Conditions

column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium acetate (pH 4.5 acetic acid): [B] acetonitrile, (75:25, A:B)
flow rate 1 mL/min
sample 100 µg/mL each in mobile phase
injection 10 µL
detector UV, 220 nm

Description

Categories Analytical Chromatography, Clinical, Pharmaceutical Analysis, antibiotics/antimicrobials, Antibiotics, Antibiotics, Antimicrobials
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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