HPLC Analysis of Pepides, Synthetic, on Discovery® BIO Wide Pore C18

HPLC Analysis of Pepides, Synthetic, on Discovery® BIO Wide Pore C18

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 2.1 mm I.D., 5 µm particles (568202-U)
column temp. 35 °C
mobile phase [A] 25 mM formic acid in water; [B] (50:50) 25 mM formic acid in water, 20 mM formic acid in acetonitrile
flow rate 0.20 mL/min
sample RP Peptide Ionic Interactions Standard, p/n RPS-I0020 (Alberta Peptide Institute)
injection 0.5 µL(~0.25 mug ea peptide)
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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