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HPLC Analysis of Peptide Mix, Complex, on Ascentis® C18

HPLC Analysis of Peptide Mix, Complex, on Ascentis® C18

Conditions

column Ascentis C18, 15 cm x 2.1 mm I.D., 5 µm particles (581304-U)
column temp. 35 C
mobile phase [A] 25 mM formic acid in water; [B]: 50:50 (v/v) 25 mM formic acid in water:25 mM formic acid in acetonitrile
gradient 10 to 64% B in 90 min
flow rate 0.2 ml/min
sample tryptic digest of carboxymethylated protein mixture 2.4 g/L in 0.1% formic acid
injection 10 µl
detector ESI (+), full scan

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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