HPLC Analysis of Peptide Mix, Complex on Ascentis® C18

HPLC Analysis of Peptide Mix, Complex on Ascentis® C18

Conditions

column Ascentis C18, 25 cm x 2.1 mm I.D., 5 µm particles (581305-U)
column temp. ambient
mobile phase [A] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:water; [B] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:acetonitrile
gradient 10 to 50% B in 80 min
flow rate 0.2 ml/min
sample tryptic digest of carboxymethylated protein mixture 1.5 g/L in 50 mM formic acid
injection 4 µl
detector ESI (+)

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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