HPLC Analysis of Peptide Separation on BIOshell™ A400 Protein C4 at Elevated Temperature

HPLC Analysis of Peptide Separation on BIOshell™ A400 Protein C4 at Elevated Temperature

Conditions

column BIOshell A400 Protein C4. 5 cm x 2.1 mm I.D., 3.4 µm particles (66824-u)
column temp. 90 °C
mobile phase (A) 95:5, 0.1% TFA in water : 0.1% TFA in acetonitrile; (B) 50:50, 0.1% TFA in water : 0.1% TFA in acetonitrile
gradient 0 to 100% B in 5 min
flow rate 0.4 mL/min
pressure 840 psi (58 bar)
sample peptide mix in 0.1% TFA
injection 0.5 µL
detector UV, 215 nm

Description

Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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