HPLC Analysis of Peptides from BSA on Proteomix® SCX-NP3, Volatile Buffer

HPLC Analysis of Peptides from BSA on Proteomix® SCX-NP3, Volatile Buffer

Conditions

column Proteomix SCX-NP1.7, 3 cm x 2.1 mm I.D., 1.7 µm particles (Z777142)
mobile phase (A) 10 mM sodium phosphate, pH 2.5; (B) A + 1 M NaCl; (C) acetonitrile
gradient 90% A, 0% B, 10% C held for 1.5 min; to 40% A, 50% B, 10% C in 18.5 min
flow rate 0.35 ml/min
pressure 5511 psi (380 bar)
sample trypsin digested bovine serum albumin (BSA)
detector UV, 214 nm

Description

Categories Analytical Chromatography, Pesticides & Herbicides
Featured Industry Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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