HPLC Analysis of Peptides on Discovery® BIO Wide Pore C5

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C5

Conditions

column Discovery BIO Wide Pore C5, 15 cm x 4.6 mm I.D., 5 µm particles (568422-U)
column temp. 30 °C
mobile phase [A]: 0.1% PFPA (pentafluoropropionic acid) in water:acetonitrile (81:19); [B]: 0.1% PFPA in water:acetonitrile (62:38)
gradient 0-100% B in 19 min
flow rate 1 mL/min
sample Cat. No. P2693
injection 10 µL~0.25 mug each peptide
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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