HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #2)

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #2)

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 µm particles (568222-U)
column temp. 30 °C
mobile phase [A] (80:20), 0.1%TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile
flow rate 1 mL/min
sample Sigma Peptide Mix (Cat. No. P2693)
injection 5 µLSigma Peptide Mix P2693 (~25 mug ea. Peptide) (~25 mug ea. peptide)
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links