HPLC Analysis of Peptides on Discovery® BIO Wide Pore C5, 0-16 Min. Window

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C5, 0-16 Min. Window

Conditions

column Discovery BIO Wide Pore C5, 15 cm x 4.6 mm I.D., 5 µm particles (568422-U)
column temp. 30 °C
mobile phase [A] (95:5), 0.1% TFA in water:0.1% TFA acetonitrile; [B] (50:50), 0.1% TFA in water:0.1% TFA in acetonitrile
gradient 0-100% B in 65 min
flow rate 1 mL/min
injection 50µL carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate (568422-U)
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links