HPLC Analysis of Peptides on Proteomix® SCX-NP3, Volatile Buffer, ELSD and UV Detection

HPLC Analysis of Peptides on Proteomix® SCX-NP3, Volatile Buffer, ELSD and UV Detection

Conditions

column Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 µm particles (Z777151)
mobile phase (A) 5 mM ammonium acetate; (B) 50 mM ammonium acetate:acetonitrile (4:1, v:v)
gradient 0 to 50% B in 20 min
flow rate 0.6 mL/min
sample 0.1 mg/mL
injection 5 µL
detector ELSD (SofTA) and UV, 280 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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