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HPLC Analysis of Peptides on BIOshell™ A160 Peptide C18 and CN

HPLC Analysis of Peptides on BIOshell™ A160 Peptide C18 and CN

Conditions

column BIOshell A160 Peptide CN, 15 cm x 4.6 mm I.D., 5 µm particles (67081-U)
column BIOshell A160 Peptide C18, 15 cm x 4.6 mm I.D., 5 µm particles (67015-U)
column temp. 40 C
mobile phase [A] water/0.1% TFA [B] acetonitrile/0.1% TFA
gradient 5-50%B in 30 minutes
flow rate 1.0 ml/min
pressure 1015 psi126 bar, 250 bar (70 bar)
sample 0.01 mg/mL in water/0.1% TFA
injection 10 µl
detector UV, 215 nm

Description

Analysis Note The availability of two distinct chemistries (C18 alkyl vs. CN) is helpful to check changes in elution order as we see for peaks # 5 and # 6 on the C18 and CN columns. As expected, peptide retention is shorter on the CN column, while peak shape is symmetrical and efficiency very high on both column types.
Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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