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HPLC Analysis of Phenolic Compounds on Ascentis® Express 2.7 μm RP-Amide

HPLC Analysis of Phenolic Compounds on Ascentis® Express 2.7 μm RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 µm particles (53929-U)
column temp. 35 C
mobile phase [A] 20 mM phosphoric acid, pH 2; [B] acetonitrile; (75:25, A:B)
flow rate 1.5 ml/min
sample 50 mg/L in mobile phase
injection 25 µl
detector UV, 270 nm

Description

Analysis Note Chromatography of Seven Phenol Compounds on Ascentis Express RPA
Categories Analytical Chromatography, Phenols
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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