HPLC Analysis of Phenolics from Rosemary on Ascentis® Express C8

HPLC Analysis of Phenolics from Rosemary on Ascentis® Express C8

Conditions

column Ascentis Express C8, 5 cm x 3 mm I.D., 2.7 µm particles (53848-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient 15 to 100% B in 8.5 min
flow rate 0.6 ml/min
sample 100 mg/L in 75 µm EDTA, 1.5 mM ascorbate, 15% acetonitrile
injection 2 µl
detector UV, 214 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C8 for the efficient separation of phenolics from rosemary.
Categories Analytical Chromatography, Phenols
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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