HPLC Analysis of Phenyl Urea Pesticides on Ascentis® Express C18 Phase

HPLC Analysis of Phenyl Urea Pesticides on Ascentis® Express C18 Phase

Conditions

column Ascentis(R) Express C18, 10 cm x 4.6 mm I.D., 2.7 µm particles (53827-U)
column temp. 30 °C
mobile phase [A] 0.025 M potassium phosphate buffer, adj. to pH = 2.5, [B] acetonitrile; (50:50, A:B)
flow rate 2.0 mL/min
pressure 300 bar
sample acetonitrile
injection 0.5 µL
detector UV 245 nm, VWD

Description

Categories Analytical Chromatography, Pesticides & Herbicides
Featured Industry Agriculture
Environmental
General description This separation illustrates the use of the highly efficient 2.7 um Ascentis Express Fused-Core C18 stationary phase in the analysis of some popular herbicides. The short run times allow analyses using isocratic conditions so that column equilibration time is not required between runs.
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
suitability application for HPLC

Materials

     
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