HPLC Analysis of Phenylalanine Methyl Ester Enantiomers on LARIHC™ CF6-P

HPLC Analysis of Phenylalanine Methyl Ester Enantiomers on LARIHC™ CF6-P


column LARIHC CF6-P, 25 cm x 4.6 mm I.D., 5 µm particles (AZYP Part No.L1001, available from Supelco/Sigma-Aldrich as a custom item)
column temp. 20 C
mobile phase [A] methanol: [B] acetonitrile: [C] acetic acid: [D] triethylamine, (70:30:0.3:0.2. A:B:C:D)
flow rate 1 ml/min
sample Phenylalanine methyl ester, 0.3 mg/mL in ethanol
injection 5 µl
detector UV, 254 nm


Categories Analytical Chromatography, Amino Acids
Featured Industry Life Science and Biopharma
General description Cyclofructans are cyclic oligosaccharides and the newest class of chiral stationary phases for HPLC, SFC, and HILIC. Invented by Prof. Daniel W. Armstrong and introduced by AZYP, LLC, the LARICH and FRULIC derivatized cyclofructan-based columns are now available through Supelco/Sigma-Aldrich. The LARIHC CF6-P chiral stationary phase (CSP) was developed as an alkyl derivatized cyclofructan 6 chiral stationary phase. It has demonstrated pronounced enantioselectivity toward all types of primary amines, such as amino alcohols, amino esters, and amino amides. Baseline separation was achieved for simple aliphatic racemic amines that contained no other functionality. Unlike all current crown ether chiral stationary phases, this new column works more effectively with organic solvents and supercritical fluids. It also appears to have great capabilities for preparative-scale separations. No other existing phase can efficiently separate primary amines as well as the LARIHC CF6-P phase. A recent study showed that this phase alone can separate 93% of tested racemic primary amines.
Legal Information LARIHC is a trademark of AZYP, LLC
suitability application for HPLC


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