HPLC Analysis of Polar Compounds on Ascentis® Express OH5 (HILIC Mode)

HPLC Analysis of Polar Compounds on Ascentis® Express OH5 (HILIC Mode)

Conditions

column Ascentis Express OH5, 10 cm x 3 mm I.D., 2.7 µm particles (53769-U)
column temp. 30 °C
mobile phase 5 mM ammonium formate (95:5 acetonitrile:water) pH 6.8
flow rate 0.6 mL/min
pressure 965 psi (66.5 bar)
sample 100 µg/mL in 25:75, water:methanol
injection 1 µL
detector UV, 254 nm

Description

Analysis Note The impact of partitioning and ion exchange in HILIC separations is demonstrated using a mixture of acidic, neutral, and basic polar compounds in this Figure. All three analytes have similar log D values, both acid and base are ionized at mobile phase pH.
Categories Analytical Chromatography, Antidiarrhoeal, Pesticides & Herbicides, Analgesics, Drugs of Abuse
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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