HPLC Analysis of Polar Compounds on Ascentis® Express RP-Amide Particles

HPLC Analysis of Polar Compounds on Ascentis® Express RP-Amide Particles

Conditions

column Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 µm particles (50773-U)
column temp. 26 °C
mobile phase [A] 20 mM potassium phosphate, pH 7 [B] acetonitrile (70:30, A:B)
flow rate 4.0 mL/min
sample 0.04-0.32 mg/mL in 50/50 water/acetonitrile
injection 5 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Amides, Amines
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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