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HPLC Analysis of Poly(ethyleneglycol) polymers on SRT® SEC-150 and SRT® SEC-300

HPLC Analysis of Poly(ethyleneglycol) polymers on SRT® SEC-150 and SRT® SEC-300

Conditions

column SRT-150, 30 cm x 7.8 mm I.D., 5 µm particles, 150 Ã… (Z777045)
column SRT-300, 30 cm x 7.8 mm I.D., 5 µm particles, 150 Ã… (Z777051)
mobile phase 150 mM phosphate Buffer, pH 7.0
flow rate 1.0 ml/min
pressure 720 psi (49.6 bar)
sample 1.5 mg/mL in mobile phase PEG 1. MW 636, 6,550, 118,000, 932,000; PEG 2. MW 2010, 42,700, 496,000 ; PEG 3. MW 232, 17,900, 271,000
injection 20 µl
detector RI(30 C)

Description

Analysis Note Although proteins are the most common applications of Sepax SRT and Zenix gel filtration columns, they can also be used to other water-soluble polymers such as the poly(ethyleneglycol) samples shown in these chromatograms. The conditions are very similar to those used for protein analysis, namely 150mM phosphate buffer at pH 7.0. Judging by the narrow peak widths all PEG polymers shwon here have rather narrow size distributions.
Categories Analytical Chromatography, Polymers, coatings, and plastics
Featured Industry Life Science and Biopharma
Legal Information SRT is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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